Shortly after the first, a second by-election took place following the resignations of another three BS Members of Parliament - S. T. Bani, Chio Cheng Thun and Kow Kee Seng, as the boycott of Parliament continued. Walkover victories, not seen since the Legislative and City Council by-elections of 1952 and 1953 respectively, returned and all three constituencies returned to PAP without pollings.

Nomination day: 1 March 1966 [Tue]

Eligible voters: 36,573

Election deposit: $500

RESULTS
Chua Chu Kang	Crawford	Paya Lebar
Total seats = 3